Title : Improving covid 19 candidate vaccine response through probiotics and micronutrient supplementation: Evaluating the role of TLR5
Abstract:
Background: This study evaluated the protective effects of two different regimens of multiple dietary supplements on the subunit protein vaccine platform in mice.
Methods: The probiotic supplement BioBoost and the PastoCovac vaccine, which contains six expressed receptor-binding domains (RBDs) and is conjugated with tetanus toxin, were administered concurrently. The safety and efficacy were assessed by determining IgG antibody titers against RBD and cytokines, measuring mRNA expression of TLR5, and evaluating clinical symptoms.
Results: Results showed that both single- and multi-dose treatment groups receiving the probiotic supplement had significantly increased IL-4, TNF-alpha, IFN-gamma, and IgG antibody titers compared to the control group, with the multi-dose treatment group exhibiting the highest levels. Moreover, the multi-dose treatment group showed significantly increased expression of TLR5 when compared to the other groups.
Conclusion: This comprehensive approach provided insights into the immune responses and demonstrated the efficacy of probiotic supplementation on the subunit protein vaccine platform.
Keywords: Probiotic; Micronutrients; Immunogenicity; PastoCovac® vaccine; COVID-19.
